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KMID : 1129720220390020043
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Korean Journal of Acupuncture
2022 Volume.39 No. 2 p.43 ~ p.53
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Effect of Paeonia Lactiflora Pallas on Atopic Dermatitis-Related Inflammation in HaCaT Cell
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Lee Hye-In
Kim Eom-Ji
Son Dong-Bin
Joo Byung-Duk
Sohn Young-Joo
Kim Eun-Young
Jung Hyuk-Sang
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Abstract
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Objectives : Paeonia lactiflora Pallas (PLP) have been reported to have pharmacological effects such as anti-inflammatory and analgesic. However, it is not yet known whether PLP extract has anti-inflammatory effect on HaCaT cells, human keratinocyte.
Methods : To confirm the anti-inflammatory effect of PLP on keratinocyte, TNF-α/IFN-γ-stimulated HaCaT cells were used. HaCaT cells were pre-treated with PLP for 1h before stimulation with TNF-α/IFN-γ. Then HaCaT cells were stimulated with TNF-α/ IFN-γ for 24 h, the cells and media were harvested to measure the inflammatory cytokines levels. Granulocyte-macrophage colony stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), interleukin 1 beta (IL-1β), and TNF-α were analyzed by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression of thymus and activation-regulated chemokines (TARC), IL-6, and IL-8 were measured by reverse transcription-polymerase chain reaction (RT-PCR). We also investigated the inhibitory mechanism of the mitogen-activated protein kinase (MAPKs) including ERK, JNK, and p38 and nuclear factor-kappaB (NF-κB) by PLP using western blot.
Results : PLP did not show cytotoxicity in HaCaT cells. In TNF-α/IFN-γ -stimulated HaCaT cells, PLP significantly inhibited the expression of GM-CSF, MCP-1 IL-1β, TNF-α, TARC and IL-6. PLP inhibited the phosphorylation of ERK and translocation of NF-κB into the nucleus.
Conclusions : These results indicate that PLP could ameliorate the TNF-α/IFN-γ-stimulated inflammatory response through inhibition of MAPK and NF-kB signal pathway. This suggests that PLP could be used beneficial agent to improve skin inflammation.
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KEYWORD
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Paeonia Lactiflora Pallas, HaCaT, pro-inflammatory cytokine, atopic dermatitis, anti-inflammatory
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